منابع مشابه
Genomics and museum specimens.
Nearly 25 years ago, Allan Wilson and colleagues isolated DNA sequences from museum specimens of kangaroo rats (Dipodomys panamintinus) and compared these sequences with those from freshly collected animals (Thomas et al. 1990). The museum specimens had been collected up to 78 years earlier, so the two samples provided a direct temporal comparison of patterns of genetic variation. This was not ...
متن کاملEvaluating hybridization capture with RAD probes as a tool for museum genomics with historical bird specimens
Laboratory techniques for high-throughput sequencing have enhanced our ability to generate DNA sequence data from millions of natural history specimens collected prior to the molecular era, but remain poorly tested at shallower evolutionary time scales. Hybridization capture using restriction site-associated DNA probes (hyRAD) is a recently developed method for population genomics with museum s...
متن کاملNon-Perishable Museum Specimens: Redefined Plastination Technique
Department of Oral Pathology & Microbiology, Kothiwal Dental College and Research Center, Mora Mustaqueem, Kanth Road, Moradabad, U.P.-244001, India Objective: The study was conducted to overcome specific limitations of formalin-preserved specimens at negligible cost. Materials and Methods: The study was conducted on museum specimens collected from the Department of General Anatomy, Kothiwal De...
متن کاملPyrosequencing for Mini-Barcoding of Fresh and Old Museum Specimens
DNA barcoding is an effective approach for species identification and for discovery of new and/or cryptic species. Sanger sequencing technology is the method of choice for obtaining standard 650 bp cytochrome c oxidase subunit I (COI) barcodes. However, DNA degradation/fragmentation makes it difficult to obtain a full-length barcode from old specimens. Mini-barcodes of 130 bp from the standard ...
متن کاملDNA Extraction from Museum Specimens of Parasitic Hymenoptera
At the same time that molecular researchers are improving techniques to extract DNA from museum specimens, this increased demand for access to museum specimens has created tension between the need to preserve specimens for maintaining collections and morphological research and the desire to conduct molecular analyses. To address these concerns, we examined the suitability of non-invasive DNA ex...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Molecular Ecology
سال: 2013
ISSN: 0962-1083
DOI: 10.1111/mec.12563